Two structural genes on different chromosomes are required for encoding the major subunit of human red cell glucose-6-phosphate dehydrogenase
Identifieur interne : 004B06 ( Main/Exploration ); précédent : 004B05; suivant : 004B07Two structural genes on different chromosomes are required for encoding the major subunit of human red cell glucose-6-phosphate dehydrogenase
Auteurs : Hitoshi Kanno [États-Unis] ; I-Yih Huang [États-Unis] ; Yuet Wai Kan [États-Unis] ; Akira Yoshida [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1989.
English descriptors
- Teeft :
- Acad, Acid, Acid sequence, Amino, Amino acid residues, Amino acid sequence, Amino acid sequence mass, Beutler, Blood cells, Cdna, Cdna clones, Cdna probe, Cell ggpd, Cell line, Chromosome, Chromosome cdna, Chromosome gene, Clone, Codon, Common junction, Dehydrogenase, Edman degradation, Encode, Experimental procedures, Fibroblast, G6pd, Genomic, Ggpd, Ggpd cdna, Ggpd protein, Ggpd subunit, Hormonal regulation, Huang, Human beings, Human chromosome, Human chromosomes, Human dehydrogenase, Human genomic, Human ggpd, Human ggpd cdna, Human liver, Human lymphoblast, Human reticulocyte, Human reticulocyte cdna library, Hybrid, Hybrid cell, Hybrid cell lines, Hybridization, Hybridized, Lymphoblast, Major ggpd subunit, Major subunit, Mass number, Mass spectrometry, Mass spectrum, Minor subunit, Mrna, Mrna components, Natl, Noncoding, Noncoding region, Noncoding regions, Northern blot hybridization, Nucleotide, Nucleotide region, Nucleotide sequence, Nucleotide sequences, Peptide, Polymerase, Polymerase chain reaction, Primer, Proc, Restriction enzymes, Reticulocyte, Single protein chain, Southern blot hybridization, Subsequent sequence, Subunit, Terminal region, Terminal sequence, Tryptic, Tryptic digests, Tryptic peptide, Tryptic peptides, Various tissues, Yoshida.
Abstract
Abstract: Structural analysis revealed the existence of two types of subunits in human red cell glucose-6-phosphate dehydrogenase. The two subunits have the same COOH region consisting of 479 amino acid residues, but their NH2-terminal regions are different in size and sequence. The minor subunit can be fully encoded by the X-linked G6PD cDNA, but the NH2-terminal region of the major subunit cannot. The cDNA and the gene for the NH2-terminal region of the major subunit were cloned and characterized. Southern blot hybridization indicated that the gene for the NH2-terminal region is on chromosome 6, not on the X chromosome. Northern blot hybridization demonstrated an existence of two separate mRNA components, one for the COOH-terminal region and the other for the NH2-terminal region. Two separate structural genes, the X-linked and chromosome 6-linked genes, must be coresponsible for encoding the single chain subunit. Either cross-translation of two mRNAs, or transpeptidation, or some other mechanism must be involved in the synthesis of human red cell G6PD.
Url:
DOI: 10.1016/0092-8674(89)90440-6
Affiliations:
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type="state">Californie</region></placeName><wicri:cityArea>Department of Laboratory Medicine and Howard Hughes Medical Institute University of California San Francisco</wicri:cityArea></affiliation></author><author><name sortKey="Yoshida, Akira" sort="Yoshida, Akira" uniqKey="Yoshida A" first="Akira" last="Yoshida">Akira Yoshida</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Biochemical Genetics Beckman Research Institute of the City of Hope Duarte</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">58</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="595">595</biblScope><biblScope unit="page" to="606">606</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Acid</term><term>Acid sequence</term><term>Amino</term><term>Amino acid residues</term><term>Amino acid sequence</term><term>Amino acid sequence mass</term><term>Beutler</term><term>Blood cells</term><term>Cdna</term><term>Cdna clones</term><term>Cdna probe</term><term>Cell ggpd</term><term>Cell line</term><term>Chromosome</term><term>Chromosome cdna</term><term>Chromosome gene</term><term>Clone</term><term>Codon</term><term>Common junction</term><term>Dehydrogenase</term><term>Edman degradation</term><term>Encode</term><term>Experimental procedures</term><term>Fibroblast</term><term>G6pd</term><term>Genomic</term><term>Ggpd</term><term>Ggpd cdna</term><term>Ggpd protein</term><term>Ggpd subunit</term><term>Hormonal 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sequences</term><term>Peptide</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Primer</term><term>Proc</term><term>Restriction enzymes</term><term>Reticulocyte</term><term>Single protein chain</term><term>Southern blot hybridization</term><term>Subsequent sequence</term><term>Subunit</term><term>Terminal region</term><term>Terminal sequence</term><term>Tryptic</term><term>Tryptic digests</term><term>Tryptic peptide</term><term>Tryptic peptides</term><term>Various tissues</term><term>Yoshida</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Structural analysis revealed the existence of two types of subunits in human red cell glucose-6-phosphate dehydrogenase. The two subunits have the same COOH region consisting of 479 amino acid residues, but their NH2-terminal regions are different in size and sequence. The minor subunit can be fully encoded by the X-linked G6PD cDNA, but the NH2-terminal region of the major subunit cannot. The cDNA and the gene for the NH2-terminal region of the major subunit were cloned and characterized. Southern blot hybridization indicated that the gene for the NH2-terminal region is on chromosome 6, not on the X chromosome. Northern blot hybridization demonstrated an existence of two separate mRNA components, one for the COOH-terminal region and the other for the NH2-terminal region. Two separate structural genes, the X-linked and chromosome 6-linked genes, must be coresponsible for encoding the single chain subunit. Either cross-translation of two mRNAs, or transpeptidation, or some other mechanism must be involved in the synthesis of human red cell G6PD.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Pennsylvanie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Kanno, Hitoshi" sort="Kanno, Hitoshi" uniqKey="Kanno H" first="Hitoshi" last="Kanno">Hitoshi Kanno</name></region><name sortKey="Huang, I Yih" sort="Huang, I Yih" uniqKey="Huang I" first="I-Yih" last="Huang">I-Yih Huang</name><name sortKey="Kan, Yuet Wai" sort="Kan, Yuet Wai" uniqKey="Kan Y" first="Yuet Wai" last="Kan">Yuet Wai Kan</name><name sortKey="Yoshida, Akira" sort="Yoshida, Akira" uniqKey="Yoshida A" first="Akira" last="Yoshida">Akira Yoshida</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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